1、Forward Genetic Screening Technology
We have licensed an unbiased forward genetic screening approach from JLP Health that uncovers the molecular on- and off-target space of drug candidates at unprecedented amino acid resolution. We use chemical mutagenesis combined with next generation sequencing and advanced bioinformatics algorithms to identify specific amino acid sites that are critical for the drug’s mode of action. Through saturating screens we are able to map the compound-target binding interface.
FEATURE 1–COMPLETENESS
Angalbio’s screening approach interrogates the complete genome in an entirely unbiased fashion. The technology identifies target structures and no prior knowledge on a potential mechanism of action is needed. It can also function as an orthogonal validation tool for existing data. Importantly, our approach includes the analysis of essential genes, that are frequently targeted by small molecules, and that are largely invisible to other screening technologies.
FEATURE 2–RESOLUTION
Through saturated screening, our core technology allows to map compound-target binding interfaces at amino acid resolution. The screen thus not only identifies the relevant target, but also the amino acids required for the interaction with the drug compound. The information obtained from this “structural biology by genetics” approach is a key for advanced and rational drug design and drug modifications.
FEATURE 3–RELEVANCE
Our screening approach requires no compound modifications but can use any relevant active substance. Importantly, the screen is performed in intact cellular systems, reflecting physiological conditions that ensure physiologically meaningful results. Finally, our technology can be applied to a wide range of different cellular systems enabling compound and indication-specific screening setups. In addition, the combination of our forward genetics target screening technology and haploid cell technology has unique application advantages in the development and screening of anti-tumor and anti-viral drug targets.
2、Haploid cell engineering technology
Angalbio has obtained the exclusive authorization in China of the haploid cell drug target screen technology and the Haplobank construction technology from JLP Health, Austria. The advantages of this technology are as follows:
1. Haploid cell technology can be used for the analysis of drug’s mode of action (MOA), overcoming the complexity and difficulty of using diploid cells, and is a game-changer for the new drug development.
2. Haplobank is constructed by haploid cell technology with repariable mutations, covering the 70% of whole genes, can be used for drug target verification or antibody specificity verification.
3. The advantage of haploid cell technology is that it can detect essential genes accounting for 10% of the whole genome, which cannot be achieved by other technologies including CRISPR/Cas9.
